Data/hora: 02/06/2024 00:49:40

Provedor de dados: 	Pesquisa Agropecuária Brasileira	
País: 	Brazil	
Título: 	Alternative genotyping method for the single nucleotide polymorphism A2959G (AF159246) of the bovine CAST gene	
Autores: 	Curi,Rogério Abdallah; Chardulo,Luis Artur Loyola; Silveira,Antônio Carlos; Oliveira,Henrique Nunes de.	
Data: 	2008-05-01	
Ano: 	2008	
Palavras-chave: 	Calpastatin gene; Meat texture; PCR-RFLP; SNP.	
Resumo: 	The objective of this work was to genotype the single nucleotide polymorphism (SNP) A2959G (AF159246) of bovine CAST gene by PCR-RFLP technique, and to report its use for the first time. For this, 147 Bos indicus and Bos taurus x Bos indicus animals were genotyped. The accuracy of the method was confirmed through the direct sequencing of PCR products of nine individuals. The lowest frequency of the meat tenderness favorable allele (A) in Bos indicus was confirmed. The use of PCR-RFLP for the genotyping of the bovine CAST gene SNP was shown to be robust and inexpensive, which will greatly facilitate its analysis by laboratories with basic structure.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500014	
Editor: 	Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira	
Relação: 	10.1590/S0100-204X2008000500014	
Formato: 	text/html	
Fonte: 	Pesquisa Agropecuária Brasileira  v.43 n.5 2008	
Fonte: 	info:eu-repo/semantics/openAccess